Uroguanylin

Clone Type

Polyclonal

Host

Rabbit

Source

Human

Swiss Prot

Q16661

Isotype

IgG

Grade

Serum

Applications

IHC WB

Crossreactivity

Shipping Temp

Blue Ice

Storage Temp

-20°C

Uroguanylin is a 16 amino acid peptide that is secreted by enterochromaffin cells in the duodenum and proximal small intestine. Guanylin acts as an agonist of the guanylyl cyclase receptor GC-C and regulates electrolyte and water transport in intestinal and renal epithelia.

Applications

Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions

Western Blot: 1:20,000 Immunohistochemistry (frozen): 1:1000 Optimal dilutions to be determined by the researcher.

Storage and Stability

Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunogen

Synthetic peptide corresponding to human Uroguanylin.

Form

Supplied as a lyophilized powder in PBS, pH 7.2. Reconstitute with 20ul/100ul sterile ddH2O.

Purity

Serum

Specificity

Recognizes human Uroguanylin.

References

1. Maake C, Auf der Maur F, Jovanovic K, Reinecke M, Hauri D, John H (2003). Occurrence and localization of uroguanylin in the aging human prostate. Histochemistry and Cell Biology 119(1): 69-76.