Urotensin II (U-II) is a cyclic heptapeptide that functions both as a potent vasoconstrictor and a neurotransmitter/ neuromodulator. U-II circulates in the plasma as a zymogen, and is activated by the urotensin converting enzyme. U-II is the endogenous ligand for the G-protein coupled receptor 14 (GPR14), recently renamed the UT receptor. Changes in the expression of U-II and its receptor have been associated with cardiovascular and renal diseases, including atherosclerosis, myocardial infarction, and diabetic nephropathy.
Applications
Suitable for use in ELISA and Radioimmunoassay. Other applications not tested.
Recommended Dilution
Radioimmunoassay: 0.24ug/ml will bind ~30% in a system using human 125I-Urotensin II as a tracer. Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide corresponding to Glu-Thr-Pro-Asp-Cys-Phe-Trp- Lys-Tyr-Cys-Val (Disulfide Bridge: Cys5-Cys10) from human Urotensin II.
Form
Supplied as a lyophilized powder from PBS, pH 7.5. Reconstitute with 200ul sterile ddH2O.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human Urotensin II. Shows 69% crossreactivity with rat Urotensin II and 10% with mouse Urotensin II. Does not crossreact with Endothelin-1 (human, porcine, rat, mouse, canine or bovine), CN-22 (human, porcine, rat), rat Adrenomedullin (aa1-50), ANF (rat, rabbit, mouse), Bradykinin, human Angiotensin II, rat BNP-45, rat BNP-32 or rat PAMP-20.
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