VPS28

Clone Type

Polyclonal

Host

Goat

Source

Human

Isotype

IgG

Grade

Affinity Purified

Applications

E IHC WB

Crossreactivity

Ca Hu Mo Po Rt

Accession #

NP_057292.1

Gene ID

51160, 66914

Shipping Temp

Blue Ice

Storage Temp

-20°C

Component of the ESCRT-I complex, a regulator of vesicular trafficking process.

Applications

Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution

ELISA: 1:1000 Western Blot: 1-3ug/ml, observed in H460, human testis and brain lysates on ~24kD bands Immunohistochemistry (Paraffin): 10ug/ml Optimal dilutions to be determined by the researcher.

Storage and Stability

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunogen

Synthetic peptide corresponding to C-ESAYNAFNRFLHA, from human VPS28, at C-terminal (NP_057292.1).

Form

Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

Purity

Purified by immunoaffinity chromatography.

Specificity

Recognizes human VPS28. Species sequence homology: Canine, mouse, porcine and rat.

References

1. Bishop N, Woodman P. TSG101/mammalian VPS23 and mammalian VPS28 interact directly and are recruited to VPS4-induced endosomes. J Biol Chem. 2001 Apr 13;276(15):11735-42.