Required for vacuolar assembly and vacuolar traffic.
Applications
Suitable for use in ELISA and Immunohistochemistry. Other applications not tested.
Recommended Dilution
ELISA: 1:16,000 Immunohistochemistry (Paraffin): 4-6ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to C-DHLKKDSQNKTLLK, from VPS41, at the internal region of the protein (NP_055211.2, NP_542198.2).
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human VPS41. Species sequence homology: Bovine, canine, mouse and rat.
References
1. McVey Ward D, Radisky D, Scullion MA, Tuttle MS, Vaughn M, Kaplan J. hVPS41 is expressed in multiple isoforms and can associate with vesicles through a RING-H2 finger motif. Exp Cell Res. 2001 Jul 1;267(1):126-34.