Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry (frozen and paraffin-embedded sections). Other applications not tested.

Immunohistochemistry: 1:20-1:50 using ABC method. We recommend incubation period of 30-60 minutes at RT. High temperature treatment of formalin-fixed tissue sections using 1mM EDTA, pH 8.0 must be performed prior to immunostaining. Optimal dilutions to be determined by the researcher.

May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

BALB/C mice were injected with a recombinant VEGF protein.

Supplied as a liquid in PBS, pH 7.2.

Purified by Protein G affinity chromatography.

Recognizes the 189, 165 and 121 isoforms of VEGF. Species Crossreactivity: human, mouse, rat and canine.