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V2118-10D VASP, Recombinant, Human CAS:

Specifications
References
Grade
Purified
Accession Number
NP_003361
Molecular Weight
37.5
EU Commodity Code
30021019
Shipping Temp
Blue Ice
Storage Temp
-20°C

Vasodilator-stimulated phosphoprotein, also known as VASP, is a member of the Ena-VASP protein family. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions.

Source
Recombinant corresponding to aa1-343 from human VASP, fused to His-tag at N-terminus expressed in E.coli.
AA Sequence
MGSSHHHHHH SSGLVPRGSH MSETVICSSR ATVMLYDDGN KRWLPAGTGP QAFSRVQIYH NPTANSFRVV GRKMQPDQQV VINCAIVRGV KYNQATPNFH QWRDARQVWG LNFGSKEDAA QFAAGMASAL EALEGGGPPP PPALPTWSVP NGPSPEEVEQ QKRQQPGPSE HIERRVSNAG GPPAPPAGGP PPPPGPPPPP GPPPPPGLPP SGVPAAAHGA GGGPPPAPPL PAAQGPGGGG AGAPGLAAAI AGAKLRKVSK QEEASGGPTA PKAESGRSGG GGLMEEMNAM LARRRKATQV GEKTPKDESA NQEEPEARVP AQSESVRRPW EKNSTTLPRM KSSSSVTTSE TQPCTPSSSD YSD
Molecular Weight
~37.5kD (363aa), confirmed by MALDI-TOF
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Recombinant, E. coli
Purity
~85% (SDS-PAGE)
Concentration
~0.5mg/ml
Form
Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0 5mM DTT, 10% glycerol, 200mM sodium chloride, 0.1mM PMSF.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
1. Laurent V., et al. (1999) J. Cell Biol. 144:1245-1258. 2. Barzik M., et al. (2005) J. Biol. Chem. 280:28653-28662
USBio References
No references available
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