Arginine vasopressin, is a nine amino acid peptide secreted from the posterior pituitary in response to reductions in plasma volume and increases is plasma osmolarity. Its release triggers water reabsorption due to the insertion of aquaporins in the membranes of kidney tubules which transport solute-free water through tubular cells and back into the blood.
Applications
Suitable for use in ELISA, Immunofluorescence and Immunohistochemistry (frozen and paraffin). Other applications not tested.
Recommended Dilution
ELISA: 1:2000-1:20,000 Optimal dilutions to be determined by the researcher.
Recommended IHC Positive Control Tissue
Hypothalamus and posterior lobe of the pituitary gland
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic arginine vasopressin conjugated to bovine thyroglobulin.
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes arginine vasopressin, also known as antidiuretic hormone. Broad reactivity expected from sequence.