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V2121-41 Mouse Anti-Verotoxin I and II (Mixed clones) (SLT-1/2, STX-1/2)

Specifications
Clone Type
Monoclonal
Host
Mouse
Source
E. coli
Isotype
IgG
Clone Number
Mixed clones
Grade
Affinity Purified
Applications
E N WB
Shipping Temp
Blue Ice
Storage Temp
-20°C
Notes
Preservative Free

Shiga-like toxins (SLTs), are also called Verotoxins. Enterohemorrhagic Escherichia coli (EHEC) strains which are primarily of serotypes 0157:H7, 026:H11 and O111:H8 have been incriminated as etiologic agents of hemorrhagic colitis and Hemolytic-uremic syndrome, a generalized disease characterized by acute renal failure, thrombocytopenia, and microangiopathic hemolytic anemia. There are several distinct E.coli SLTs. SLT-I and SLT-II are produced by EHEC. SLT-I and Shiga toxin share >99% deduced amino acid sequence homology, whereas SLT-I and SLT-II share about 60% deduced amino acid sequence homology. SLT-I and SLT-II are antigenically distinct. The protein structure of the toxin consists of two domains: the A polypeptide that inhibits protein synthesis by targeting ribosomes, and the B polypeptide pentamer that binds to the eukaryotic cell receptor globotriaosylceramide (Gb3) leading to receptor-mediated endocytosis.

Applications
Suitable for use in ELISA, Western Blot and Toxin Neutralization. Other applications not tested.
Recommended Dilutions
ELISA (Capture): 20ug/ml Western Blot: 1ug/ml Toxin Neutralization: 5ug/ml neutralizes Vero Cell toxicity of E.coli O157:H7. Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Form
Supplied as a lyophilized powder from 5mM phosphate buffer, pH 7.3. No preservatives added. Reconstitute with 1ml sterile ddH20.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes E. coli Shigatoxin 1 and 2.
Conjugates
Pricing
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