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V2122-01D Chicken Anti-Vimentin (FLJ36605, VIM) (Not for Export EU)

Specifications
Clone Type
Polyclonal
Host
Chicken
Source
Rat
Swiss Prot
M14144
Isotype
IgY
Grade
Serum
Applications
IHC
Crossreactivity
Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C

Human vimentin is a 53,521 dalton protein (466 amino acids) expressed in many cell types of mesodermal origin, and is one of the intermediate filament types that form the cytoskeleton. In the embryonic nervous system, however, vimentin is expressed by migrating neural crest cells forming the PNS, and by neural stem cells of the CNS. In some forms of glioblastoma, vimentin is re-expressed by the tumor cells and serves as a general indicator of their undifferentiated state. Immunohistochemical staining for Vimentin is also characteristic of sarcomas (of neural, muscle and fibroblast origin) compared to carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin antibodies are thus of value in the differential diagnosis of undifferentiated neoplasms and malignant tumors.

Applications
Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Immunohistochemistry: 1:5000 Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies
I1906: IgY, Chicken Pab Rb x Ch I1906-10: IgY, Chicken (HRP) Rb x Ch I1906-12: IgY, Chicken (FITC) Rb x Ch I1906-14: IgY, Chicken (AP) Rb x Ch
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant rat vimentin purified from E. coli
Form
Supplied as a liquid.
Purity
Serum
Specificity
Recognizes rat Vimentin.
Conjugates
Pricing
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