Vimentin is a member of the intermediate filament protein family. Intermediate filaments are an important structural feature of eukaryotic cells. They, along with microtubules and actin microfilaments, make up the cytoskeleton. Despite the fact that most intermediate filaments exist as stable structures, in fibroblasts, vimentin take on a dynamic structure.
Applications
Suitable for use in ELISA, Western Blot, Flow Cytometry and mmunohistochemistry. Other applications not tested.
Recommended Dilutions
ELISA: 0.05-0.2ug/ml Western Blot: 0.5-1ug/ml Immunohistochemistry: 5-10ug/ml Flow Cytometry: 1-3ug for 1x10e6 cells Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide corresponding to a region within residues 400-500 of human vimentin, conjugated to KLH (Swiss Prot: P08670). Species Sequence Homology: mouse and rat
Form
Supplied as a lyophilized powder from PBS, pH 7.4, 0.02% sodium azide. Reconstitute with 80ul sterile ddH2O.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes endogenous levels of human vimentin protein
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