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V2122-01N Mouse Anti-Vimentin (VIM)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1
Clone Number
65E
Grade
Purified
Applications
IHC WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C

Vimentin is the major subunit protein of the intermediate filaments of mesenchumal cells, and has a molecular weight of 57kD. Immunohistochemical staining for Vimentin is thus characteristic of sarcomas (of neural, muscle and fibroblast origin) compared to carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin antibodies are thus of value in the differential diagnosis of malignant tumors, generally used with a panel of other antibodies including those recognizing cytokeratins, lymphoid markers, S100, desmin and neurofilaments.

Applications
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000 Immunohistochemistry: frozen Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Electrophoretically purified vimentin from cultured human fibroblast
Form
Supplied as a liquid in PBS, 1% BSA, 0.09% sodium azide.
Purity
Purified
Specificity
Recognizes human Vimentin. Stains the 57kD vimentin band in immunoblots performed on a lysate of normal human lymphocytes. It does not stain mouse or rat vimentin.
References
1. Heikinheimo K. et al. (1991) J. Pathol. 164, 145-150. |2. Virtanen I. et al. (1988) Lab. Invest. 59, 5, 649-656. |3. Lehto V.P. and Virtanen I., (1986) Cancer Rev. 6, 1-20. |4. Virtanen I. et al. (1984) Ann. N.Y. Acad. Sci. 455, 635-648. |5. Miettinen M. et al. (1984) Ultrastruct. Pathol. 7, 83-107.
USBio References
No references available
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