Vimentins are class-III intermediate filaments specific to mesenchymal tissue. Vimentin is an important cytoskeletal component responsible for maintaining cell integrity and has a probable role in the intracellular transport of proteins such as lipoproteins between the nucleus and plasma membrane. Immunohistochemical staining for Vimentin is characteristic of sarcomas.
Applications
Suitable for use in Western Blot and Immunohisochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 0.5-1ug/ml Immunohistochemistry (Paraffin and Formalin): 1.5-2.5ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Pig eye lens vimentin.
Form
Supplied as a lyophilized powder from 1.2% sodium acetate, 2% BSA, 0.2% sodium azide. Reconstitute with 1ml of PBS.
Purity
Purified by affinity chromatography.
Specificity
Recognizes porcine Vimentin at 54kD. Species Crossreactivity: human, mouse, rat.