Visinin-like protein 1

Clone Type

Polyclonal

Host

Sheep

Source

Human

Isotype

IgG

Grade

Affinity Purified

Applications

E WB

Crossreactivity

Shipping Temp

Blue Ice

Storage Temp

-20°C

Notes

Preservative Free

Visinin-like protein 1 (VILIP-1) is a cytoplasmic protein of low molecular weight (~22kD) consisting of 191aa residues. It belongs to the visinin/recoverin subfamily of neuronal calcium sensor proteins involved in calcium-dependent signal transduction mechanisms in neurons. It is found primarily in the brain and nerve cells, but it also has a peripheral distribution in liver, lung, kidney, spleen, pancreas and colon.

Applications

Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions

Optimal dilutions to be determined by the researcher.

Amino Acid Sequence

MKHHHHHHAS MGKQNSKLAP EVMEDLVKST EFNEHELKQW YKGFLKDCPS GRLNLEEFQQ LYVKFFPYGD ASKFAQHAFR TFDKNGDGTI DFREFICALS ITSRGSFEQK LNWAFNMYDL DGDGKITRVE MLEIIEAIYK MVGTVIMMKM NEDGLTPEQR VDKIFSKMDK NKDDQITLDE FKEAAKSDPS IVLLLQCDIQ K

Storage and Stabillty

Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunogen

Recombinant protein corresponding to 191aa from human Visinin-like protein 1 and 10 extra aa, N-terminal His-tag expressed in E. coli (P62760).

Form

Supplied as a lyophilized powder in PBS, pH 7.2. No preservative added. Reconstitute with 100ul sterile dH2O. Let the lyophilized pellet dissolve completely.

Purity

Purified by immunoaffinity chromatography.

Specificity

Recognizes human Visinin-like protein 1.

References

1. Verbeek MM, et al. Clin Chem. 2008 Oct;54 (10):1589-91. 2. Ladenson JH, et al. Clin Chem. 2006 Sep;52 (9):1713-21. 3. Braunewell KH, et al. Neurobiol Dis. 2008 Oct;32 (1):162-75. 4. Braunewell KH, et al. J Cell Sci. 2005 Jun 1;118 (Pt 11):2495-505. 5. Chen KC, et al. J Pept Sci. 2009 Feb;15 (2):89-94. 6. Ladenson JH, et al. Clin Chem. 2008 Oct;54 (10):1617-23. 7. Anand R, et al. J Biol Chem. 2002 Nov 1;277 (44):41872-8. 8. Wheeler MB, et al. J Biol Chem. 2006 Aug 4;281 (31):21942-53. 9. Schnurra I, et al. Neurobiol Dis. 2001 Oct;8 (5):900-9. 10. Burgoyne RD. The neuronal calcium- sensor proteins. Biochimica et Biophysica Acta. 2004. 11. Braunewell KH. VILIP-1. UCSD-Nature Molecule Pages. 2009. 12. Klein-Szanto AJ, et al. PLoS One. 2008;3 (2):e1698. 13. Klein-Szanto AJ, et al. Cell Tissue Res. 2009 Feb;335 (2):301-16.

USBio References

No references available

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V2124-26B Sheep Anti-Visinin-like protein 1 (VSNL1, VILIP-1)

Clone Type

Host

Source

Isotype

Grade

Applications

Crossreactivity

Shipping Temp

Storage Temp

Notes

References

USBio References

Conjugates

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