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W0477-01 Mouse Anti-WC4 (SWC7)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Bovine
Isotype
IgG1
Clone Number
CC55
Grade
Affinity Purified
Applications
FC IHC IP
Crossreactivity
Bo Po Sh
Shipping Temp
Blue Ice
Storage Temp
-20°C

Mouse anti-bovine WC4 antibody, clone CC55 recognizes the bovine WC4 cell surface antigen, a ~90kDa molecule expressed by a subpopulation of B cells in peripheral blood and lymphoid tissues (Howard et al. 1993). It is suggested that WC4 like SWC7 in pigs is the bovine orthologue of human CD19 (Naessens and Howard 1991, Ikebuchi et al. 2013).

Applications
Suitable for use in Flow Cytometry, Immunohistochemistry (frozen), and Immunoprecipitation. Other applications not tested.
Recommended Dilution
Flow Cytometry: 1:50-1:100; 10ul labels 10e6 cells in 100ul Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Bovine WC4.
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes the bovine WC4 at ~90kD. Species Crossreactivity: porcine, sheep. Note: Antibody reactivity and working conditions may vary between species.
References
1. Howard, C.J. et al. (1991) Summary of workshop findings for leukocyte antigens of cattle. Vet. Immunol. Immunopathol. 27: 21-27. 2. Denham, S. et al. (1994) Monoclonal antibodies recognizing differentiation antigens on porcine B cells. Vet. Immunol. Immunopathol. 43: 259-267. 3. Naessens, J. et al. (1997) Nomenclature and characterization of leukocyte differentiation antigens in ruminants. Immunol. Today 8:365-368. 4. Boersma. et al. (2001) Summary of workshop findings for porcine B-cell markers. Vet. Immunol. Immunopathol. 80: 63-78.
USBio References
No references available
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