WC1

Clone Type

Monoclonal

Host

Mouse

Source

Bovine

Swiss Prot

P30205

Isotype

IgG2a

Clone Number

CC15 (10F583)

Grade

Affinity Purified

Applications

FC IHC IP

Crossreactivity

Bo Gt Sh

Shipping Temp

Blue Ice

Storage Temp

-20°C

Bovine WC1 is a 215/300kD antigen expressed on the majority of gamma/delta T lymphocytes. These cells also express low levels of CD5, but are negative for other B and T cell markers.

The WC1 antigen is reported to be involved in a number of processes, including activation of gamma/delta T cells and the development of a Th1-biased acquired immune response.

Applications

Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications not tested.

Recommended Dilutions

Flow Cytometry: 1:25-1:200; 10ul labels 10e6 cells in 100ul Immunohistochemistry (Paraffin): Requires protein digestion pre-treatment of paraffin sections. e.g. trypsin or pronase. Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher.

Hybridoma

NS1 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunogen

Bovine lymphocytes.

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

Purity

Purified by Protein G affinity chromatography from tissue culture supernatant.

Specificity

Recognizes bovine WC1. Species Crossreactivity: sheep and goat.

References

1. Howard, C. J. et al. (1989) In vivo depletion of BoT4 (CD4) and of non-T4/T8 lymphocyte subsets in cattle with monoclonal antibodies. Eur. J. Immunol. 19: 757-764. 2. Clevers, H. et al. (1990) Identification of a bovine surface antigen uniquely expressed on CD4- CD8- T cell receptor gamma/delta+ T lymphocytes. Eur. J. Immunol. 20: 809-817. 3. Gutierrez, M. et al. (1999) The detection of CD2+, CD4+, CD8+, and WC1+ T lymphocytes, B cells and macrophages in fixed and paraffin embedded bovine tissue using a range of antigen recovery and signal amplification techniques. Vet. Immunol. Immunopathol. 71: 321-334. 4. Brodersen, R. et al. (1998) Analysis of the immunological cross reactivities of 213 well characterized monoclonal antibodies with specificities against various leukcocyte surface antigens of human and 11 animal species. Vet. Immunol. Immunopathol. 64: 1-13. 5. Winkler, M. T. C. et al. (2000) Persistence and Reactivation of Bovine Herpesvirus 1 in the Tonsils of Latently Infected Calves. J. Virol. 74: 5337-5346.

USBio References

No references available

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W0481-74C Mouse Anti-WC1 (Antigen WC1.1, CD163L1, CD163 Molecule-like 1)

Clone Type

Host

Source

Swiss Prot

Isotype

Clone Number

Grade

Applications

Crossreactivity

Shipping Temp

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References

USBio References

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