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W0481-74F Mouse Anti-WC1 (FITC)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Sheep
Swiss Prot
Q9BDF1
Conjugate
FITC
Isotype
IgG1
Clone Number
10F582
Grade
Affinity Purified
Applications
FC
Crossreactivity
Bo Gt Sh
Shipping Temp
Blue Ice
Storage Temp
-20°C

WC1 cell surface antigen is a ~215kD glycoprotein, expressed by a population of gamma/delta T lymphocytes (Wijngaard et al.1992).

Applications
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilution
Flow Cytometry: Neat; 10ul labels 10e6 cells in 100ul Optimal dilutions to be determined by the researcher.
Hybridoma
NS-1 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Sheep thymocytes.
Form
Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes sheep WC1. Species Crossreactivity: bovine, goat.
References
1. Mackay, C. et al. (1986) Three distinct subpopulations of sheep T lymphocytes. Eur. J. Immunol. 16: 19 - 25. 2. Mackay, C. et al. (1987) A monoclonal antibody to the p220 component of sheep LCA identifies B cells and a unique lymphocyte subset. Cell. Immunol. 110: 46 -55.
USBio References
No references available
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