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W0481-74G Mouse Anti-WC1 (Antigen WC1.1, CD163L1, CD163 Molecule-like 1) (FITC)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Bovine
Conjugate
FITC
Isotype
IgG2a
Clone Number
10F583
Grade
Affinity Purified
Applications
FC
Crossreactivity
Bo Gt Sh
Shipping Temp
Blue Ice
Storage Temp
-20°C

Bovine WC1 is a 215/300kD antigen expressed on the majority of gamma/delta T lymphocytes. These cells also express low levels of CD5, but are negative for other B and T cell markers.

The WC1 antigen is reported to be involved in a number of processes, including activation of gamma/delta T cells and the development of a Th1-biased acquired immune response.
Applications
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilution
Flow Cytometry: Neat-1:10; 10ul labels 10e6 cells in 100ul Optimal dilutions to be determined by the researcher.
Hybridoma
NS1 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Bovine lymphocytes.
Form
Supplied as a liquid in PBS, 1% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes bovine WC1. Species Crossreactivity: sheep, goat.
References
1. Howard, C. J. et al. (1989) In vivo depletion of BoT4 (CD4) and of non-T4/T8 lymphocyte subsets in cattle with monoclonal antibodies. Eur. J. Immunol. 19: 757-764. 2. Clevers, H. et al. (1990) Identification of a bovine surface antigen uniquely expressed on CD4- CD8- T cell receptor gamma/delta+ T lymphocytes. Eur. J. Immunol. 20: 809-817. 3. Gutierrez, M. et al. (1999) The detection of CD2+, CD4+, CD8+, and WC1+ T lymphocytes, B cells and macrophages in fixed and paraffin embedded bovine tissue using a range of antigen recovery and signal amplification techniques. Vet. Immunol. Immunopathol. 71: 321-334. 4. Brodersen, R. et al. (1998) Analysis of the immunological cross reactivities of 213 well characterized monoclonal antibodies with specificities against various leukcocyte surface antigens of human and 11 animal species. Vet. Immunol. Immunopathol. 64: 1-13. 5. Winkler, M. T. C. et al. (2000) Persistence and Reactivation of Bovine Herpesvirus 1 in the Tonsils of Latently Infected Calves. J. Virol. 74: 5337-5346.
USBio References
No references available
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