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W1002-70D Rabbit Anti-WBSCR14 (Williams Beuren Syndrome Chromosome Region 14, Williams-Beuren Syndrome Chromosome Region 14 Protein, Carbohydrate Responsive Element Binding Protein, CHREBP, MIO, MLX Interacting Protein-like, MLXIPL, Mlx Interactor, MONDOB, WS Basic Helix-loop-helix Leucine Zipper Protein, WS bHLH, WS-bHLH) (Biotin)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Conjugate
Biotin
Isotype
IgG
Grade
Affinity Purified
Applications
IF IHC IP WB
Crossreactivity
Hu Mo Rt
Accession #
NP_116569.1, isoform 1/alpha]
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze

ChREBP is a transcription factor involved in activating genes that encode enzymes of fatty acid biosynthesis in liver and adipose. This protein may have relevance to lipid metabolism, obesity, and type II diabetes.

Applications
Suitable for use in Western Blot, Immunoprecipitation, Immunofluorescence and Immunohistochemistry (formalin-fixed paraffin-embedded sections). Other applications not tested.
Recommended Dilution
Western Blot: a band is seen corresponding to the theoretical molecular weight of human ChREBP of ~95kD. Immunoprecipitation: chromatin Immunofluorescence: 1:100-1:500 Optimal dilution determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
An C-terminal synthetic peptide made to the human ChREBP protein sequence (between residues 800-852).
Form
Supplied as a liquid. No preservatve added. Labeled with Biotin.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human ChREBP. Species Crossreactivity: mouse and rat. There is 83% homology of the immunogen with the mouse ChREBP protein.
References
1. Xu, J., et al. GENES: STRUCTURE AND REGULATION: Regulation of Rat Hepatic L-Pyruvate Kinase Promoter Composition and Activity by Glucose, n-3 Polyunsaturated Fatty Acids, and Peroxisome Proliferator-activated Receptor- alpha Agonist. J. Biol. Chem., 281: 18351-18362, Jul 2006.|2. Dentin, R., et al. Liver-Specific inhibition of ChREBP improves hepatic steatosis and Insulin Resistance in ob/ob Mice. Diabetes. 55: 2159-2170, 2006.|3. Wang, Y., et al. Regulation of hepatic fatty acid elongase and desaturase expression in diabetes and obesity. J. Lipied Res. 47: 2028-2041, 2006.|4. Erhuma, A. M., Salter, A. M., Sculley, D. V., Langley-Evans, S. C., and Bennett, A. (2007) Prenatal exposure to a low protein diet programmes disordered regulation of lipid metabolism in the ageing rat, 00605.02006.|5. Aneja KK, Guha P, Shilpi RY, et al. The presence of distal and proximal promoters for rat mitochondrial glycerol-3-phosphate acyltransferase. Arch. Biochem. Biophy. 2008;470:35-43.|6. Wang, Y., Torres-Gonzalez, M., Tripathy, S., et al. Elevated hepatic fatty acid elongase-5 activity affects multiple pathways controlling hepatic lipid and carbohydrate composition. J. Lipid Res. 2008. 1538-1552.|
USBio References
No references available
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