The trypsin-like protease, NS3, and its co-factor, NS2b, play an essential role in replication and are therefore potential targets for treatment and prevention of West Nile Virus disease.
Applications
Suitable for use in Direct ELISA and Western Blot. Other applications not tested.
Recommended Dilutions
Western Blot: 1ug/ml. Using recombinant Viral wnvNS3 Protease Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding Gly1506-Leu1689 from West Nile Virus NS3 Protease, expressed as a fusion protein with co-factor NS2L, expressed in E. coli.
Form
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Purity
Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Specificity
Recognizes West Nile Virus NS3 Protease. Recognizes an epitope in the NS3 domain.