WIZ

Clone Type

Polyclonal

Host

Goat

Source

Human

Isotype

IgG

Grade

Affinity Purified

Applications

E WB

Crossreactivity

Hu Mo Rt

Accession #

NP_067064.2

Gene ID

58525, 22404, 314598

Shipping Temp

Blue Ice

Storage Temp

-20°C

May link EHMT1 and EHMT2 histone methyltransferases to the CTBP corepressor machinery. May be involved in EHMT1-EHMT2 heterodimer formation and stabilization.

Applications

Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution

ELISA: 1:2000 Western Blot: 0.3-1ug/ml, observed in human brain (cerebellum) lysates on ~75kD bands Optimal dilutions to be determined by the researcher.

Storage and Stability

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunogen

Synthetic peptide corresponding to C-RSYIQGGRPFTKKFR, from human WIZ, at the internal region of the protein (NP_067064.2).

Form

Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

Purity

Purified by immunoaffinity chromatography.

Specificity

Recognizes human WIZ. Species sequence homology: Mouse and rat.

References

1. Rathert P, Dhayalan A, Murakami M, Zhang X, Tamas R, Jurkowska R, Komatsu Y, Shinkai Y, Cheng X, Jeltsch A. Protein lysine methyltransferase G9a acts on non-histone targets. Nat. Chem. Biol. 2008 Jun 4(6): 344-6.

USBio References

No references available

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W1106-90 Goat Anti-WIZ (Protein Wiz, Widely-interspaced Zinc Finger-containing Protein, Zinc Finger Protein 803, ZNF803)

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