Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Western Blot: 1ug/ml Immunohistochemistry (Paraffin): 8-25ug/ml; Used to detect WIF-1 in immersion fixed paraffin-embedded human breast sections. Optimal dilutions to be determined by the researcher.

Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Recombinant protein corresponding to Gly29-Trp379 from human WIF-1, expressed in NSO cells

Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with sterile PBS.

Purified by Protein G affinity chromatography from hybridoma culture supernatant

Recognizes human Wnt Inhibiting Factor 1. Shows less than 5% crossreactivity with recombinant mouse WIF-1.