XIAP-associated factor 1 (XAF1) binds to X-linked inhibitor of apoptosis (XIAP) and antagonizes the anticaspase activity of XIAP. XAF1 is expressed in all adult and fetal tissues but is frequently down-regulated in cancer cell lines. At least 7 isoforms of XAF1 are produced by alternative splicing.
Applications
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 1ug/ml. Immunohistochemistry (Paraffin and Formalin): 0.5-1ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide corresponding to aa283-301, QEKCRWLASSKGKQVRNFS to the C-terminus of human XIAP-associated factor 1.
Form
Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstitute with 100ul of sterile dH20.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human XIAP-associated factor 1. Species Crossreactivity: rat. Sequence homology: mouse.
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