YAP

Clone Type

Polyclonal

Host

Rabbit

Source

Human

Swiss Prot

P46937

Isotype

IgG

Grade

Affinity Purified

Applications

ChIP IC IF IHC IP WB

Crossreactivity

Ca Hu Mo Rt Ze

Shipping Temp

Blue Ice

Storage Temp

-20°C

YAP (Yes-Associated Protein) is a founding member of the WW domain family of proteins, transcriptional co-activator and oncogene. Its gene is transcriptionally activated in squamous cell carcinoma cells. As one of several genes of 1q22 amplicon, YAP was recently implicated in liver and breast cancer. Overexpression of YAP gene in MCF10A mammary cell line induces oncogenic transformation.

Applications

Suitable for use in ChIP, Immunohistochemistry, Immunoprecipitation, Western Blot and Immunofluorescence/Immunocytochemistry. Other applications not tested.

Recommended Dilutions

Immunofluorescence (IC): 2-10ug/ml Immunohistochemistry (Frozen/Paraffin): 1:50-1:200 Western Blot: 1:1000. Detects 65kD band using transfected HEK 293 lysate as sample. Immunoprecipitation: 2-10ug Optimal dilutions to be determined by the researcher.

Positive Control

Brain and kidney lysates and HEK 293 cell lysate

Storage and Stability

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunogen

Recombinant protein corresponding to human YAP protein expressed in bacteria. Cellular Localization: Nuclear.

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

Purity

Purified by immunoaffinity chromatography.

Specificity

Recognizes human YAP1. Species Crossreactivity: mouse, rat, canine and zebrafish.

References

1. Sudol, M., et al. Characterization of the Mammilian YAP Gene and Its Role in Defining a Novel Protein Module, the WW Domain. JBC. 14733-14741 (1995). 2. Komuro, A., et al. WW Domain-containing Protein YAP Associates with WrbB-4 and Acts as a Co-transcriptional Activator for the Carboxyterminal Fragment of ErB4 that Translocates to the Nucleus. JBC. 33334-33341 (2003). 3. Zender, L., et al. Identification and Validation of Oncogenes in Liver Cancer Using an Integrative Oncogenomic Approach. Cell. 125:1230-3 (2006). [Western blot, Immunohistochemistry] 4. Dong, G., et al. Molecular Profiling of Transformed and Metastatic Murine Squamous Carcinoma Cells by Differential Display and cDNA Microarray Reveals Altered Expression of Multiple Genes Related to Growth, Apoptosis, Angiogenesis, and the NF-kappaB Signal Pathway. Cancer Res. 61:4797-808 (2001). 5. Overholtzer, M., et al. Transforming Properties of Yap, a Candidate Oncogene of the Chromosome 11q22 Amplicon. PNAS. 103:12405-10 (2006).

USBio References

No references available

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- Coupon Code

Y1200-01D Rabbit Anti-YAP (65kD Yes-associated Protein, YAP 65, YAP65, YAP-65, Yes Associated Protein 1 65kD, Yes-associated Protein 1, YAp 1, YAP1, YAP2)

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