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Y1200-01G Goat Anti-YAP (65kD Yes-associated Protein, YAP 65, YAP65, YAP-65, Yes Associated Protein 1 65kDa, Yes-associated Protein 1, YAp 1, YAP1, YAP2)

Specifications
References
Clone Type
Polyclonal
Host
Goat
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Accession #
NP_006097.1
Shipping Temp
Blue Ice
Storage Temp
-20°C
yes-associated protein 2, Yes-associated protein 1, 65kD, Yes-associated protein 1, 65 kD, YAP65, YAP2, YAP, Yes-associated protein 1, 65kDa

This gene encodes the human ortholog of chicken YAP protein which binds to the SH3 domain of the Yes proto-oncogene product. This protein contains a WW domain that is found in various structural, regulatory and signaling molecules in yeast, nematode, and mammals, and may be involved in protein-protein interaction.

Applications
Suitable for use in ELISA and Western Blot. Other applications have not been tested.
Recommended Dilutions
Peptide ELISA Titer: 1:8000 Western Blot: 1-3ug/ml ~90kD band observed in lysates of cell line HepG2 (calculated MW of 48.8kD according to NP_006097.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to C-TYHSRDESTDS, from the internal region of human YAP1 (NP_006097.1).
Form
Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Purity
Purified by peptide affinity chromatography.
Specificity
Recognizes human YAP1. Species sequence homology: mouse, rat, canine.
References
1. Zender L, Spector MS, Xue W, Flemming P, Cordon-Cardo C, Silke J, Fan ST, Luk JM, Wigler M, Hannon GJ, Mu D, Lucito R, Powers S, Lowe SW. Identification and validation of oncogenes in liver cancer using an integrative oncogenomic approach.Cell. 2006 Jun 30;125(7):1253-67. PMID: 16814713
USBio References
No references available
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