Yeast Nitrogen Base (YNB) media have been prepared according the formulae of Wickerham (1943-51) and Burkholder (1943). Their research on the nutritional requirements of yeast strains resulted in the formulation of YNB. YNB is a well defined composition of salts, vitamins, amino acids and a nitrogen source for a vigorous growth of Sacharomyces cerevisiae.
| L-Histidine•HCl | 10mg |
| DL-Methionine | 20mg |
| DL-Tryptophan | 20mg |
| p-Aminobenzoic acid | 200ug |
| Biotin | 2ug |
| Boric Acid | 500ug |
| Copper Sulfate | Absent |
| Ferric Chloride | Absent |
| Folic Acid | 2ug |
| Inositol | 2000ug |
| Manganese Sulfate | 400ug |
| Niacin | 400ug |
| D-Pantothenic Acid•Ca | 400ug |
| Potassium Iodide | 100ug |
| Pyridoxine•HCl | 400ug |
| Riboflavin | 200ug |
| Sodium Molybdate | 200ug |
| Thiamine•HCl | 400ug |
| Zinc Sulfate | 400ug |
| Ammonium Sulfate | 5g |
| Calcium Chloride | 0.1g |
| Magnesium Sulfate | 0.5g |
| Potassium Phosphate Monobasic | 1g |
| Sodium Chloride | 0.1g |
| Total: 6.76g/L |
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