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Y2021 Yeast Nitrogen Base w/ AA & w/ AS, w/o Carbohydrate w/o Fe, Cu (YNB) (Powder)

Specifications
References
Grade
Molecular Biology Grade
EU Commodity Code
38210000
Shipping Temp
RT
Storage Temp
RT/4°C
Wickerham formula with amino acids & with ammonium sulfate

Yeast Nitrogen Base (YNB) media have been prepared according the formulae of Wickerham (1943-51) and Burkholder (1943). Their research on the nutritional requirements of yeast strains resulted in the formulation of YNB. YNB is a well defined composition of salts, vitamins, amino acids and a nitrogen source for a vigorous growth of Sacharomyces cerevisiae.

Application
For carbon assimilation tests in the characterization and identification of yeasts.
Note
The addition of a carbon source like glucose, galactose or raffinose is required.
Appearance
White to pale yellow, homogeneous, free flowing powder
Solubility
Colorless to pale yellow, clear, complete
pH
As Reported
Directions per Liter
Dissolve 6.76g in 800-900ml of ddH2O stirring gently until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.
Storage and Stability
Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Media Formulation
Components per liter
L-Histidine•HCl10mg
DL-Methionine20mg
DL-Tryptophan20mg
p-Aminobenzoic acid200ug
Biotin2ug
Boric Acid500ug
Copper SulfateAbsent
Ferric ChlorideAbsent
Folic Acid2ug
Inositol2000ug
Manganese Sulfate400ug
Niacin400ug
D-Pantothenic Acid•Ca400ug
Potassium Iodide100ug
Pyridoxine•HCl400ug
Riboflavin200ug
Sodium Molybdate200ug
Thiamine•HCl400ug
Zinc Sulfate400ug
Ammonium Sulfate5g
Calcium Chloride0.1g
Magnesium Sulfate0.5g
Potassium Phosphate Monobasic1g
Sodium Chloride0.1g
Total: 6.76g/L
References
1. Difco Manual Tenth Edition, pp.1136-1141(1984). 2. Ronald M. Atlas, Handbook of Microbiological Media, Lawrence C. Parks, ed., CRC Press (1993).
USBio References
No references available
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