Yeast Nitrogen Base (YNB) media have been prepared according the formulae of Wickerham (1943-51) and Burkholder (1943). Their research on the nutritional requirements of yeast strains resulted in the formulation of YNB. YNB is a well defined composition of salts, vitamins, amino acids and a nitrogen source for a vigorous growth of Sacharomyces cerevisiae.
L-Histidine•HCl | 10mg |
DL-Methionine | 20mg |
DL-Tryptophan | 20mg |
p-Aminobenzoic acid | 200ug |
Biotin | 2ug |
Boric Acid | 500ug |
Copper Sulfate | Absent |
Ferric Chloride | Absent |
Folic Acid | 2ug |
Inositol | 2000ug |
Manganese Sulfate | 400ug |
Niacin | 400ug |
D-Pantothenic Acid•Ca | 400ug |
Potassium Iodide | 100ug |
Pyridoxine•HCl | 400ug |
Riboflavin | 200ug |
Sodium Molybdate | 200ug |
Thiamine•HCl | 400ug |
Zinc Sulfate | 400ug |
Ammonium Sulfate | 5g |
Calcium Chloride | 0.1g |
Magnesium Sulfate | 0.5g |
Potassium Phosphate Monobasic | 1g |
Sodium Chloride | 0.1g |
Total: 6.76g/L |
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