Yeast Nitrogen Base (YNB) media have been prepared according the formulae of Wickerham (1943-51) and Burkholder (1943). Their research on the nutritional requirements of yeast strains resulted in the formulation of YNB. YNB is a well defined composition of salts, vitamins, amino acids and a nitrogen source for a vigorous growth of Sacharomyces cerevisiae.
| Yeast Nitrogen Base w/AS, w/o Vitamins, AA |
| Ammonium Sulfate | 5g |
| Boric Acid | 500ug |
| Copper Sulfate | 40ug |
| Ferric Chloride | 200ug |
| Manganese Sulfate | 400ug |
| Potassium Iodide | 100ug |
| Sodium Molybdate | 200ug |
| Zinc Sulfate | 400ug |
| Calcium Chloride | 0.1g |
| Magnesium Sulfate | 0.5g |
| Potassium Phosphate Monobasic | 1g |
| Sodium Chloride | 0.1g |
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