USBio Logo

Y2030-02 Yeast Nitrogen Base w/AS, w/o AA, Vitamins (YNB) (Powder)

Specifications
References
Grade
Molecular Biology Grade
EU Commodity Code
38210000
Shipping Temp
RT
Storage Temp
RT/4°C
Wickerham formula without amino acids, vitamins & with ammonium sulfate

Yeast Nitrogen Base (YNB) media have been prepared according the formulae of Wickerham (1943-51) and Burkholder (1943). Their research on the nutritional requirements of yeast strains resulted in the formulation of YNB. YNB is a well defined composition of salts, vitamins, amino acids and a nitrogen source for a vigorous growth of Sacharomyces cerevisiae.

Appearance
White to light yellow, homogeneous, free-flowing powder
Solubility
Colorless to pale yellow, clear, complete
pH
As Reported
Directions per Liter
Dissolve 6.70 g in 800-900ml of ddH2O, stirring gently until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).
Storage and Stability
Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Media Formulation
Yeast Nitrogen Base w/AS, w/o Vitamins, AA
Components per liter
Nitrogen Source
Ammonium Sulfate5g
Trace Elements
Boric Acid500ug
Copper Sulfate40ug
Ferric Chloride200ug
Manganese Sulfate400ug
Potassium Iodide100ug
Sodium Molybdate200ug
Zinc Sulfate400ug
Inorganic Salts
Calcium Chloride0.1g
Magnesium Sulfate0.5g
Potassium Phosphate Monobasic1g
Sodium Chloride0.1g
References
1. Difco Manual Tenth Edition, pp.1136-1141(1984). 2. Ronald M. Atlas, Handbook of Microbiological Media, Lawrence C. Parks, ed., CRC Press (1993).
USBio References
No references available
Pricing
Order
Proceed to Checkout
Cart Summary
ProductSizeListYour PriceQtyExt Price
Subtotal:Subtotal:
Subtotal:Subtotal:
Total Coupon Savings:Total Coupon Savings:()
Your cart is currently empty.
- Coupon Code
Recently Viewed
  • Contact Us

    Visit our technical library or contact our support staff to answer your questions.

    Telephone:
    1-800-520-3011

    Library | Contact

    Distributors

    For customers outside of the United States, please use one of our many distributors.

    View Distributors

    Payment Methods

    We accept the following payment methods as well as pay-by-invoice.

    MasterCard Visa PayPal
    © 2023-2024 United States Biological - All Rights Reserved