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Y2035-01 Yeast Nitrogen Base w/o AA & w/o AS, w/o Vitamins (YNB) (Powder)

Specifications
References
Grade
Molecular Biology Grade
EU Commodity Code
38210000
Shipping Temp
RT
Storage Temp
RT/4°C
Wickerham formula without amino acids & without ammonium sulfate & without Vitamins

Yeast Nitrogen Base (YNB) media have been prepared according the formulae of Wickerham (1943-51) and Burkholder (1943). Their research on the nutritional requirements of yeast strains resulted in the formulation of YNB. YNB is a well defined composition of salts, vitamins, amino acids and a nitrogen source for a vigorous growth of Sacharomyces cerevisiae.

Y2035-01 is based upon the formulation of YNB except that all vitamins are omitted. The medium is used to test the requirement of essential vitamins.
Application: For carbon assimilation tests in the characterization and identification of yeasts.
Appearance
White to tan, homogeneous, free-flowing powder
Solubility
Colorless to yellow, clear, complete
pH
As Reported
Directions per Liter
Dissolve 1.70 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to 1L. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).
Storage and Stability
Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Media Formulation
Components per liter
Trace Elements
Boric Acid500ug
Copper Sulfate40ug
Ferric Chloride200ug
Manganese Sulfate400ug
Potassium Iodide100ug
Sodium Molybdate200ug
Zinc Sulfate400ug
Inorganic Salts
Calcium Chloride0.1g
Magnesium Sulfate0.5g
Potassium Phosphate Monobasic1g
Sodium Chloride0.1g
Total1.701 g/L
References
1. Difco Manual Tenth Edition, pp.1136-1141(1984). 2. Ronald M. Atlas, Handbook of Microbiological Media, Lawrence C. Parks, ed., CRC Press (1993).
USBio References
No references available
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