Yeast Nitrogen Base (YNB) media have been prepared according the formulae of Wickerham (1943-51) and Burkholder (1943). Their research on the nutritional requirements of yeast strains resulted in the formulation of YNB. YNB is a well defined composition of salts, vitamins, amino acids and a nitrogen source for a vigorous growth of Sacharomyces cerevisiae. Y2035-05 is based upon the formulation of YNB except that all vitamins are omitted. The medium is used to test the requirement of essential vitamins.
| Ammonium Sulfate | 5000 |
| Dextrose | Absent |
| L-Histidine•HCl | 10 |
| DL-Methionine | 20 |
| DL-Tryptophan | 20 |
| Biotin | Absent |
| D-Pantothenic Acid•Ca | Absent |
| Folic Acid | Absent |
| Inositol | Absent |
| Niacin | Absent |
| p-Aminobenzoic acid | Absent |
| Pyridoxine•HCl | Absent |
| Riboflavin | Absent |
| Thiamine•HCl | Absent |
| Boric Acid | 0.5 |
| Copper Sulfate | 0.04 |
| Ferric Chloride | 0.2 |
| Manganese Sulfate | 0.4 |
| Potassium Iodide | 0.1 |
| Sodium Molybdate | 0.2 |
| Zinc Sulfate | 0.4 |
| Calcium Chloride | 100 |
| Magnesium Sulfate | 500 |
| Potassium Phosphate Mono | 1000 |
| Sodium Chloride | 100 |
| Total | 6.7g/L |
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