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Z0133 Goat Anti-ZAG (Zinc-a2-Glycoprotein 1)

Specifications
Clone Type
Polyclonal
Host
Goat
Source
Human
Swiss Prot
Q5XKQ4
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C

ZAG (zinc-a2-glycoprotein; also ZA2G) is a 40kD, secreted member of the MHC class I family of proteins. It is produced by adipocytes and various epithelial cells that generate exocrine-type secretions. ZAG is reported to stimulate lipid breakdown and thus may play a role in lipid homeostasis. Mature human ZAG is 278 amino acids (aa) in length. It contains one MHC class I antigen region (aa 26-201) and a C2-type Ig-like domain (aa 207-292). Two alternate splice forms exist; one shows a 66 aa substitution for the C-terminal 30 aa, and a second shows a nine Lys substitution for aa 151-298. Mature human ZAG is 60% aa identical to mouse ZAG.

Applications
Suitable for use in Direct ELISA and Western Blot. Other applications not tested.
Recommended Dilution
Direct ELISA: 0.5 -1.0ug/ml detection limit for rhZAG is ~ 0.3ng/well. Western Blot: 1.0ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Mouse myeloma cell line NS0-derived recombinant human ZAG,|Gln21-Ser298
Form
Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human ZAG.
Conjugates
Pricing
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