Suitable for use in Immunohistochemistry, Flow Cytometry, Western Blot and Direct ELISA. Other applications not tested.

Immunohistochemistry: 8ug/ml detects BACE1 in paraffin-embedded human Alzheimer’s brain tissue sections. Flow Cytometry: 25ug/ml; 10ul labels 1-2.5x10e5 cells in a total reaction volume < 200ul. The binding of unlabeled monoclonal antibodies may be visualized by adding 10ul of a 25ug/ml stock solution of a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. Western Blot: 2ug/ml detects human BACE1 in cell or tissue extracts under reducing conditions only. Direct ELISA: 0.5-1.0ug/ml. The detection limit is ~1ng/well. Optimal dilutions to be determined by the researcher.

Blotting Buffer: 25mM Tris, pH 7.4, 0.15M sodium chloride, 0.05% Tween-20 Blocking Solution: 5% nonfat dry milk in Blotting Buffer Antibody Solution: 5% nonfat dry milk in Blotting Buffer

Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Recombinant protein corresponding to Thr22-Tyr460 of human BACE1 expressed in NSO cells.

Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 1ml sterile PBS.

Purified by Protein G affinity chromatography from hybridoma culture supernatant.

Recognizes human BACE1. Species Crossreactivity: mouse.