Suitable for use in Western Blot, Flow Cytometry, Immunocytochemistry and ELISA. Other applications not tested.

Western Blot: 1ug/ml using recombinant human Integrin beta 1/CD29 under non-reducing conditions only Flow Cytometry: 0.2mg/ml 10ul labels 1-2.5x 10e5 cells in ≤200ul. Immunocytochemistry: 10ug/ml Optimal dilutions to be determined by the researcher.

Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Recombinant protein corresponding to Gln21-Asp728 (predicted) of human Integrin Beta-1 expressed in CHO cells (P05556).

Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with 1ml sterile, ddH2O or sterile 40-50% glycerol.

Purified by Protein G affinity chromatography.

Recognizes human CD29. Species Crossreactivity: porcine and equine