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C2381-08 Mouse Anti-CD29 (Integrin beta 1)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Swiss Prot
P05556
Isotype
IgG1,k
Clone Number
k2D5
Grade
Affinity Purified
Applications
E FC IC IF WB
Crossreactivity
Hu
Accession #
NP_002202
Shipping Temp
Blue Ice
Storage Temp
-20°C

CD29, also known as Integrin beta 1, is the beta subunit found in the integrin families, forming a heterodimer integrin receptor through non-covalent bonding with various integrin alpha subunits. Integrin receptors are involved in the regulation of a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. Interaction between integrins and the extracellular matrix lead to activation of signal transduction pathways and regulation of gene expression.

Applications
Suitable for use in Immunofluorescence/Immunocytochemistry, ELISA, Western Blot, and Flow Cytometry. Other applications not tested.
Recommended Dilutions
Immunofluorescence (IC): 1:100 Flow Cytometry: 2-5ug for 1x10e6cells Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to aa34-141 from human CD29, expressed in E. coli.
Form
Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 10% glycerol.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human CD29.
References
1. Li H, et al., (2003) Blood 102(10):3652-3657. 2. Carlos A, et al., (2000) J cell Biol 149(6):1263-1274. 3. Ines M P, et al., (1994) J Biol Chem 269:6124-6132.
USBio References
No references available
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