Applications
Suitable for use in Immunohistochemistry and Immunofluorescence. Other applications not tested.
Recommended Dilutions
Immunofluorescence: 1:200 Immunohistochemistry (PAP or ABC): 1:500 Optimal dilutions to be determined by the researcher.
Amino Acid Sequence
His-Ser-Gln-Gly-Thr-Phe-Thr-Ser-Asp-Tyr-Ser-Lys-Tyr-Leu-Asp-Ser-Arg-Arg-Ala-Gln-Asp-Phe-Val-Gln-Trp-Leu-Met-Asn-Thr
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide corresponding to human Glucagon.
Form
Supplied as a lyophilized powder. Reconstitute with sterile dH2O.
Specificity
Recognizes human Glucagon. Species Crossreactivity: rat, mouse, porcine and bovine. Does not react with human, rat or mouse Glucagon-Like Peptide 1, human Glucagon-Like Peptide 2, human Insulin, Somatostatin, human Amylin, human CGRP, rat Amylin, human PPP or Pro-Insulin.