CD271, also known as p75NTR, TNFRSF16, p75(NTR), Gp80-LNGFR, and NGFR, is a type I transmembrane protein with a MW of 75 kD. It is expressed by many cell types including neurons, Schwann cells, mesenchymal stem/stromal cells, follicular dendritic cells and melanocytes. The extracellular portion contains four TNFR-Cys repeats that form the binding domain for its ligands (NGF, BDNF, NTF3, and NTF4). The intracellular portion of CD271 contains a death domain, which interacts with TRAF2, TRAF4, TRAF6, PTPN13 and RANBP9, to promote cell apoptosis, and to regulate cell differentiation and neurogenesis.
Applications
Suitable for use in Flow Cytometry. Though not tested, according to references additional reported applications include: Immunoprecipitation (ref. 1), Western Blot (ref. 4), Immunohistochemistry staining (frozen or paraffin embedded tissue sections) (ref. 1 and 2) and Blocking of NGF binding (ref. 1). Other applications not tested.
Recommended Dilution
Flow Cytometry: 5ul/10e6 cells in 100ul Optimal dilution determined by the researcher.
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
WM245 melanoma cells.
Form
Supplied as a liquid in PBS, pH 7.2, BSA, 0.09% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
Purity
Purified by affinity chromatography.
Specificity
Recognizes human p75NTR (low affinity neurotrophin receptor). Species Crossreactivity: African Green, Baboon, Cynomolgus, Rhesus
TDS
NCBI
UniProt
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