Pancreatic Polypeptide Y, HGNC-9327
Gene ID: 5539. This gene belongs to the NPY family and it encodes a protein that is synthesized as a 95 aa polypeptide precursor in the pancreatic islets of Langerhans. It is cleaved into two peptide products; the active hormone of 36 aa and an icosapeptide of unknown function. The hormone acts as a regulator of pancreatic and gastrointestinal functions and may be important in the regulation of food intake. Plasma level of this hormone has been shown to be reduced in conditions associated with increased food intake and elevated in anorexia nervosa. In addition, infusion of this hormone in obese rodents has shown to decrease weight gain.
Applications
Suitable for use in Immunohistochemistry and Immunofluorescence. Other applications not tested.
Recommended Dilution
Immunofluorescence: 1:70 Immunohistochemistry (PAP or ABC): 1:70 Optimal dilutions to be determined by the researcher.
AA Sequence
Ala-Pro-Leu-Glu-Pro-Val-Tyr-Pro-Gly-Asp-Asn-Ala-Thr-Pro-Glu-Gln-Met-Ala-Gln-Tyr-Ala-Ala-Asp-Leu-Arg-Arg-Tyr-Ile-Asn-Met-Leu-Thr-Arg-Pro-Arg-Tyr-NH2
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide corresponding to human Pancreatic Polypeptide.
Form
Supplied as a lyophilized powder. Reconstitute with sterile dH2O.
Specificity
Recognizes human Pancreatic Polypeptide. % Cross-reactivity with Peptides: Pancreatic Polypeptide (Human): 100%; Pancreatic Polypeptide (Rat): 10%; Somatostatin: 0%; Insulin (Human): 0%; Glucagon (Human): 0%; Pancreatic Polypeptide (Avian): 0%; Pancreatic Polypeptide (Salmon): 0%; alpha-hCGRP: 0%; Amylin Amide (Human): 0%; Amylin (Rat): 0%
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