Suitable for use in ELISA, Western Blot and Immunohistochemistry Other applications not tested.

Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL. ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well. Immunohistochemistry: 2-20ug/ml. Optimal dilutions to be determined by the researcher.

Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

17 amino acid peptide sequence near the carboxy terminal region of rat VGAT (KLH coupled). Cellular Localization: Cytoplasmic

Supplied as a lyophilized powder from PBS, 0.05% sodium azide.

Purified using a peptide-Sepharose column.

Recognizes rat VGAT. The peptide shows no significant homology with other known proteins. The VGAT antibody crossreactivity in other species is not known. The appropriate control immunogenic peptides are also available to confirm specificity of antibodies.