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236772 Vitamin D2 USP/BP/CP CAS: 50-14-6

Specifications
References
CAS Number
50-14-6
Grade
USP
Molecular Formula
C₂₈H₄₄O
EU Commodity Code
38220090
Shipping Temp
RT
Storage Temp
4°C
Vitamin D2; Calciferol; Ergosterol; Ergocalciferol; (3β,5Z,7E,22E)-9,10-Secoergosta-5,7,10(19),22-tetraen-3-ol; Calciferol; Ercalciol; Oleovitamin D2; Radiostol; Radsterin; (+)-Vitamin D2; Viosterol; Vio D; Vitavel-D

Synonyms
Ergocalciferol
Molecular Formula
C₂₈H₄₄O
Molecular Weight
396.70
CAS No
50-14-6
MDL No
MFCD00166988
Grade
USP/BP/CP
Potency
~40,000,000 IU/g
Purity (HPLC)
97.0-103.0%
Appearance
White to off-white crystalline powder
Melting Point
115-119 °C
Optical Rotation
+103 to +106º
Solubility
Acetone, Chloroform, DMSO, Ethanol, Methanol
Odor
Odorless
Infrared Spectrum
Conforms to structure
Reducing Substances
≤ 20 ppm
Organic Volatile Impurities
Conforms to USP
Warnings
Highly toxic! Light sensitive
Storage Temp
Store at 0-8 °C
Additional Notes
Dissolve about 0.5mg of Vitamin D2 into 5mL chloroform, add 0.3mL acetic anhydride and 0.1mL sulfuric acid, shake and mix vigorously. The mixture is initially produced bright red color, and then rapidly changes violet and finally blue to green.
Dissolve 10mg of vitamin D2 into 2mL of 90% ethanol, add 2mL solution of digitalis saponin and incubate for 18h. No precipitation or cloud should be observed.
Contains no Ergosterol.
References
Knudson, B., et al.: J. Biol. Chem., 124, 287 (1938), Huber, B., et al.: J. Biol. Chem., 149, 125 (1943), Crowfoot, D., et al.: Nature, 162, 608 (1948), Suda, et al.: Biochem. Biophys. Res. Commun., 35, 182 (1969)
USBio References
No references available
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