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277796 Fibrin (Bovine) CAS: 9001-31-4

Specifications
CAS Number
9001-31-4
Swiss Prot
P02672, P02676, P12799
Grade
Molecular Biology Grade
MDL Number
MFCD00163624
Molecular Formula
C5H11N3O2
Molecular Weight
145.16
EU Commodity Code
38220090
Shipping Temp
RT
Storage Temp
-20°C
Factor I

Fibrinogen has been used in studies of haemostatic therapy in surgical and massive trauma patients. These studies have shown that fibrinogen may prove to be more superior in stopping blood loss when compared to using fresh frozen plasma (FFP).

Fibrinogen is an acute phase protein that is part of the coagulation cascade of proteins. The end result of the cascade is the production of thrombin that converts fibrinogen to fibrin. Thrombin rapidly proteolyzes fibrinogen, releasing fibrinopeptide A. The loss of this small peptide is not sufficient to make the resulting fibrin molecule insoluble, but it tends to form complexes with adjacent fibrin and fibrinogen molecules. Thrombin then cleaves a second peptide, fibrinopeptide B, from fibrin and the fibrin monomers formed then polymerize spontaneously to form an insoluble gel. The polymerized fibrin is held together by noncovalent and electrostatic forces and stabilized by the transamidating enzyme, factor XIIIa, that is produced by the action of thrombin on factor XIII. The insoluble fibrin aggregates (clots) and aggregated platelets then block the damaged blood vessel and prevent further bleeding. The amount of fibrinogen in the plasma can serve as a nonspecific indicator of whether or not an inflammatory process is present in the body. Fibrinogen from any mammalian source will be cleaved by thrombin from any mammalian source.
Source
Fibrinogen from bovine plasma
CAS Number
9001-31-4
Protein
65-85%
Clottable Protein
≥75%
Molecular Weight
α-chain 63.5kD β-chain 56kD γ chain 47kD (~4% carbohydrate content) Soluble dimer 340kD
Appearance
Supplied as an off-white to white powder. Contains ~10% sodium citrate and ~15% sodium chloride.
Solubility
Colorless to light yellow, slightly hazy to hazy
Water Content
≤10%
Reconstitution
The optimal way to solubilize fibrinogen is to layer it on top of warm (37ºC) saline, as fibrinogen will not dissolve in water. The saline concentration can be in the range of 0.85-0.9%. The fibrinogen-saline solution can be gently agitated, but it must not be vortexed. The fibrinogen will slowly dissolve to give a hazy solution. Fibrinogen may be sterile-filtered, but may not go through a 0.1um filter. A 0.2um filter is suggested, with positive pressure using a syringe and "button" filter. Vacuum filtration should not be used, since this will lead to breakdown of the molecule during the filtration.
Storage and Stability
Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile saline. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Toxicity and Hazards
 All products should be handled by qualified personnel only, trained in laboratory procedures.
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