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027939 S100 Calcium Binding Protein A8 (S100A8) BioAssay™ ELISA Kit (Human)

Specifications
Brand
BioAssay™
Kit Type
Sandwich ELISA
Tests
96
Sample Volume
100ul
Sensitivity
31pg/ml
Detection Method
Colorimetric
Detection Range
78-5000pg/ml
Sample Matrix
serum, plasma, tissue culture
EU Commodity Code
38220000
UN DOT Shipping
UN2796 PGII
Shipping Temp
Blue Ice
Storage Temp
4°C/-20°C

S100 Calcium Binding Protein A8 (S100A8) BioAssay™ ELISA Kit (Human) is a sandwich enzyme immunoassay for in vitro quantitative measurement of S100A8 in human serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.

Detection Range
78-5000pg/ml
Sensitivity
35pg/ml
Intra-Assay CV
≤10%
Inter-Assay CV
≤12%
Kit Components
*027939A: Microtiter Strips, 1x96 wells, Pre-coated, ready to use *027939B: Standard, 2x1 vial  027939C: Standard Diluent, 1x20ml *027939D: Detection Reagent A, 1x120ul  *027939E: Detection Reagent B, 1x120ul  027939F: Assay Diluent A, 2X, 1x6ml 027939G: Assay Diluent B, 2X, 1x6ml 027939H: TMB Substrate, 1x9ml 027939K: Stop Solution, 1x6ml 027939L: Wash Buffer, 30X, 1x20ml
Precaution
The Stop Solution (027939K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Storage and Stability
Store *027939A, *027939B, *027939D and *027939E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
Assay Procedure Summary
1. Prepare all reagents, samples and standards; 2. Add 100ul standard or sample to each well. Incubate 2 hours at 37°C. 3. Add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C. 4. Aspirate and wash 3 times. 5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C. 6. Aspirate and wash 5 times. 7. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37°C. 8. Add 50ul Stop Solution. Read at 450nm immediately.
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