PARK2; PRKN; PRKN2; Parkinson disease protein 2; Parkinson juvenile disease protein 2; ubiquitin E3 ligase PRKN
The Parkin Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Parkin protein expression profile in cells. The kit can be used for measuring the relative amounts of Parkin in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Parkin.
Sensitivity
>5000cells/well
Kit Components
472747A: Microtiter Strips, 1x96 wells 472747B: TBS, 10X, 1x24ml 472747C: Quenching Buffer, 1x24ml 472747D: Blocking Buffer, 1x50ml 472747E: Wash Buffer, 15X, 1x50ml *472747F: Parkin Pab, 100X, 1x60ul *472747G: GAPDH Mab, 100X, 1x60ul 472747H: IgG Pab (HRP), 1x6ml 472747J: IgG Mab (HRP), 1x6ml 472747K: Primary Antibody Diluent, 1x12ml 472747L: Substrate, 1x12ml 472747M: Stop Solution (2N Sulfuric Acid), 1x12ml 472747N: Crystal Violet Solution, 1x6ml *472747P: SDS Solution, 1x24ml
Storage and Stability
Store all components at 4°C. Reconstituted *472747F and *472747G are stable for 2 weeks at 4°C.. *472747P should be stored at RT or wrmed up to RT if stored at 4ºC. Other components are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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