Dulbecco’s MEM is the most widely used modification of BME. It contains a 4-fold higher concentration of amino acids and vitamins. Non-essential amino acids and certain essential trace elements were added. The bicarbonate concentration was increased. The standard formula for DMEM is with 1000mg/ml glucose. DMEM was originally developed for the culture of mice embryonic cells. Today, it finds a broad application of serum free culture of normal and transformed mouse and chicken cells.
| Calcium Chloride•2H2O | 0.265 |
| Ferric Nitrate•9H2O | 0.0001 |
| Magnesium Sulfate | 0.09767 |
| Potassium Chloride | 0.40 |
| Sodium Chloride | 6.40 |
| Sodium Phosphate Mono | 0.109 |
| L-Arginine•HCl | 0.084 |
| L-Cystine Absent | |
| Glycine | 0.03 |
| L-Glutamine Absent | |
| L-Histidine•HCl•H2O | 0.042 |
| L-Isoleucine | 0.105 |
| L-Leucine | 0.105 |
| L-Lysine•HCl | 0.146 |
| L-Methionine | 0.03 |
| L-Phenylalanine | 0.066 |
| L-Serine | 0.042 |
| L-Threonine | 0.095 |
| L-Tryptophan | 0.016 |
| L-Tyrosine•2Na•2H2O | 0.10379 |
| L-Valine | 0.094 |
| Choline Chloride | 0.004 |
| Folic Acid | 0.004 |
| myo-Inositol | 0.0072 |
| Niacinamide | 0.004 |
| D-Pantothenic Acid•Ca | 0.004 |
| Pyridoxal•HCl | 0.004 |
| Riboflavin | 0.0004 |
| Thiamine•HCl | 0.004 |
| Glucose Absent | |
| Phenol Red Absent | |
| Sodium Pyruvate Absent |
| Total: | 8.26g/liter |
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