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S5350-31 Sorbitol Dehydrogenase, aa1-357, Recombinant, Human (SDH, SORD, SORD1, L Iditol 2 Dehydrogenase, L-iditol 2-dehydrogenase) CAS:

Specifications
References
Grade
Purified
Accession Number
AAH25295
Molecular Weight
40.4
EU Commodity Code
30021019
Shipping Temp
Blue Ice
Storage Temp
-20°C

SORD, also known as L-iditol 2-dehydrogenase or SORD1, is a 357aa member of the zinc-containing alcohol dehydrogenase family. It is widely expressed with highest expression in kidney and in the lens of the eye. SORD enzymatically catalyzes the zinc-dependent interconversion of polyols, such as sorbitol and xylitol, to their respective ketoses.

Source
Recombinant corresponding to aa1-357 of human SORD 6x his tagged, expressed in E.coli.
AA Sequences
MGSSHHHHHH SSGLVPRGSH MAAAAKPNNL SLVVHGPGDL RLENYPIPEP GPNEVLLRMH SVGICGSDVH YWEYGRIGNF IVKKPMVLGH EASGTVEKVG SSVKHLKPGD RVAIEPGAPR ENDEFCKMGR YNLSPSIFFC ATPPDDGNLC RFYKHNAAFC YKLPDNVTFE EGALIEPLSV GIHACRRGGV TLGHKVLVCG AGPIGMVTLL VAKAMGAAQV VVTDLSATRL SKAKEIGADL VLQISKESPQ EIARKVEGQL GCKPEVTIEC TGAEASIQAG IYATRSGGTL VLVGLGSEMT TVPLLHAAIR EVDIKGVFRY CNTWPVAISM LASKSVNVKP LVTHRFPLEK ALEAFETFKK GLGLKIMLKC DPSDQNP
Enzyme Activity
Specific activity is > 20,000 pmol/min/ug, and is defined as the amount of enzyme that catalyze D-fructose to D-sorbitol per minute at pH 7.5 at 37°C.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Recombinant, E. coli
Purity
~90% (SDS-PAGE)
Concentration
~0.5mg/ml
Form
Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 0.2M sodium chloride, 5mM DTT, 20% glycerol.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
1. Baker MA., et al. (2010) Proteomics. 10(3):482-95. 2. Sorger D., et al. (2009) Nucl Med Biol. 36(1):17-27.
USBio References
No references available
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